|
KMID : 1234420090370040405
|
|
Korean Journal of Microbiololgy and Biotechnology
2009 Volume.37 No. 4 p.405 ~ p.407
|
|
|
Expression of a Bacillus subtilis Mannanase Gene in Corynebacterium lactofementum.
|
|
Yoon Ki-Hong
|
|
|
Abstract
|
|
|
|
A Bacillus subtilis mannanase gene was subcloned into an Escherichia coli - Corynebacterium lactofermentum shuttle vector pHE83, and the resultant plasmid pHE83M was transferred into an endogenous plasmid-free strain of C. lactofermentum. Mannanase produced by the recombinant C. lactofermentum (pHE83M) was secreted extracellulary at the level of 86%, and the remaining activity of mannanase was detected in the cell-free extract. The maximum mannanase productivity of the recombinant strain reached 8.1 unit/mL in the culture filtrate of LB medium. According to the zymogram of mannanase on SDS-PAGE, it was found that the mannanase produced by the recombinant C. lactofermentum could be maintained stably with a migration identical to the mannanase produced by the parental strain, B. subtilis WL-3.
|
|
|
KEYWORD
|
|
|
Bacillus subtilis WL-3, Corynebacterium lactofermentum, expression, mannanase
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|